factors affecting saccharomyces cerevisiae

factors affecting saccharomyces cerevisiae Chapter 1: Introduction Research Questions: What is the effect of differing temperatures on Saccharomyces cerevisiae population growth? What is the effect of differing pH levels on Saccharomyces cerevisiae population growth? What is the effect of differing glucose concentrations on Saccharomyces cerevisiae population growth? The yeast Saccharomyces cerevisiae is a single celled fungus that reproduces asexually by budding or division. It is one of the most well studied eukaryotic model organisms in both molecular and cell biology. S. cerevisiae is a very good type of yeast for biological studies owing to the rapid growth (doubling time 1.5-2 hours at 30 Â °C), the dispersed cells and the ease of replica planting. Moreover is a non-pathogenic organism, so can be handled fearlessly with only little precautions. Also large amounts of commercial bakers yeast are available with result being an easy and cheap source for biochemical studies. S. cerevisiae has round to ovoid cells between 3-8ÃŽÂ ¼m in diameter. 1.2 Respiration In biology, respiration is defined as: the process by which the energy in food molecules is made available for an organism to do biological work (Kent, 2000; p.100). It is also called Cellular respiration. This process of cellular respiration happens in every living cell as it is the only way to obtain energy in a form that will be usable for the cell, so it can carry out the functions of movement, growth and reproduction (ibid). The food in yeasts must be obtained as they cannot produce it on their own. For yeasts, a very good source of energy is sugars. All strains of S. cerevisiae can metabolize glucose (a hexose sugar), maltose and trehalose. 1.3 Types of Respiration There are two main types of respiration that take place within a cell: Anaerobic respiration (without oxygen) and Aerobic respiration (with oxygen). S. cerevisiae can metabolize sugars in both ways, but in this research the cultures of yeast were exposed to air hence to oxygen, so aerobic respiration was mainly the way that yeast cells grew and reproduced. 1.4 Enzymes Thousands of chemical reactions are carried out within a cell. These reactions most of the times occur in a very slow rate. For that reason living organisms make biological catalysts which are called enzymes and speed up these reactions. Enzymes are globular proteins which act as catalysts of chemical reactions (Allot, 2007; p.18. Also cells can control which reaction occurs in their cytoplasm by making some enzymes and not others. Enzymes achieve to increase the rate of a reaction by decreasing the activation energy (the minimum amount of energy required for a reaction to occur) (Greenwood. Et al. 2007; p.167) of the substrate or the substrates, when binding to the activation site (Greenwood. et al. 2007; p.114). Enzymes are sensitive molecules with very specific structure which enables them to carry out specific reactions. This structure including the active site can be damaged by various conditions and substrates. This damage is called denaturation and is usually permanent for an enzyme and if denaturation is occurred the enzyme can no longer carry out its function. As a result when enzymes are required to catalyze a reaction, is necessary that they have appropriate conditions. Different enzymes have different ideal conditions called optimum. The factors that affect the enzyme activity are: the temperature, the pH, the substrate concentration. The effect of temperature, pH and substrate concentration upon the enzyme activity which affects the growth of S. cerevisiae yeast cells are studied in this research. 1.5 Effect of Temperature As the temperature is increased in an enzyme-catalysed reaction, the rate of reaction is increased up to maximum in a specific temperature. This is called optimum temperature. The optimum temperature of Saccharomyces cerevisiae is 30o- 32oC. Above this temperature the rate starts to drop rapidly. This is due to the high energy that causes vibration inside the enzyme with result the bonds which maintain the structure of enzyme to break. This causes denaturation and the active site can no longer fit the substrate. 1.6 Effect of pH (hydrogen ion concentration) Most of the enzymes operate effectively in a small range of pH values. Between these pH values there is an optimum pH value in which the enzyme activity is the highest. The optimum pH of Saccharomyces cerevisiae is 5.5. Acids and alkalis cause denaturation of the structure of the enzyme by breaking mainly hydrogen and ionic bonds with result the substrate cant fit the active site. Furthermore the charges of the amino acids within the active site are affected by pH changes, so the enzyme is not able to form an enzyme-substrate complex. Above and below the optimum pH the enzymatic activity hence the rate is reduced considerably. 1.7 Effect of Substrate concentration In an enzyme-catalysed reaction the rate increases in direct proportion to the substrate concentration. The optimum glucose concentration of Saccharomyces cerevisiae is 2%. At low substrate concentrations, the rate of enzymatic activity increases sharply as the substrate increases. This occurs due to the more frequent collisions between the substrate molecules and the unoccupied active sites. On the other hand, at high substrate concentrations the biggest part of the active sites have been occupied with result when increasing the substrate concentration there is little effect on the rate of enzymatic activity. 1.8 Purpose of the research The purpose of this research that is carried out to examine the growth of S. cerevisiae yeast cells in different factors and various conditions is not so in order to discover which are the optimum conditions in each factor (as this species of S. cerevisiae is very well studied and examined professionally because of its usage in food industry) but to examine in real conditions and not theoretical ones how altering the environment of a cell affects the way its enzymes work and help the whole cell to live and divide in all of the previously ways that were mentioned in the introduction part.

Othello and Film “O”

Despite the shift in time and place, the two texts Shakespeare’s Othello and Tim Blake Nelson’s film ‘O’ explores timeless ideas such as jealousy and illusion versus reality. These ideas transcend through time and still remain constant in modern society. To achieve universal themes represented in Othello, Tim Blake Nelson uses a variety of different techniques such as camera angles and Verdi’s Opera music to effectively achieve such themes and values in his film to suit his 21st century adolescent audiences.On the other hand, Shakespeare have originated the plot by using literacy devices such as imagery, soliloquies and asides to suit his contemporary Elizabethan audience. In Shakespeare’s Othello, the theme jealousy is evident throughout the play. The composer uses techniques such as imagery and characterisation to convey this idea effectively. The protagonist Othello was characterised as a general, a man who has high social standards, ration al, logical and a military man who is known as the â€Å"Valiant† and â€Å"more fail than black†.This has shaped a strong comparison towards the end of the play when the Moor who is perplexed and enraged by jealousy for Desdemona, calling her â€Å"Impudent Strumpet! †. Othello is a man of reason, and it is not until the proof of the handkerchief does the audience see his decline into distrust. With Iago’s understanding of human nature and their characteristics, Iago was able to turn their honesty against themselves. Othello’s mind became so corrupted by Iago that Othello got lose of his mind â€Å"Lie with her? Lie on her?.. Handkerchief! – Oh devil!† that the once calm and honourable figure was unfortunately lost to the â€Å"green eyed monster which doth mock the meat it feeds on†.Shakespeare uses imagery to describe jealousy as a monster, effectively highlighted the destructive nature of jealousy. In a different form the f ilm â€Å"O† also represents the theme of jealousy effectively. Tim Blake Nelson conveys jealousy effectively through techniques such as super imposition, a variety of non diegetic (Verdi’s opera representing tragedy at the opening scene of doves) and a range of different angled camera shots to convey his theme of jealousy.The composer utilizes the popular medium of cinema as well as the use of colloquial language, slangs and profanities to suit his contemporary adolescent audiences. Oden having similar characteristics to Othello is naive and easily trusting who fall into the deception of Hugo. The use of non diegetic sounds as well as superimposition was imposed to symbolise jealousy. Tim Blake Nelson used such technique in the â€Å"willow† scene, the lyrics â€Å"Even the sun goes down Heroes eventually die† are all forecasts of the tragic death of Desi.The sex scene when Oden looks into the mirror and saw Mike’s face looking straight back at hi m smiling â€Å"evilly† gives the audience a look into Oden’s mind of jealousy and an indication of corruption of Oden’s mind. Iago is a success model in reflecting the theme â€Å"illusion verses reality†. Shakespeare employs techniques such as soliloquies and dramatic irony to effectively present the theme to his audience. In the beginning of the play, audiences are quickly known the â€Å"devil† of the story is Iago. Deception than arises in Othello when the protagonist constantly referred Iago as â€Å"the honest Iago†.However the audience is aware that Iago is indeed not honest at all, from quotes â€Å"I am not what I am† and â€Å"In following him I follow but myself† is an indication of the double-faced Iago. Iago is seen as the most loyal friend to Othello, Iago helped Othello to â€Å"prove† the unfaithfulness of Desdemona and evidenced Othello being cuckold by Desdemona. The effective use of asides creates dra matic irony for the audiences as Othello’s naivety and trust in Iago is evident. These scenes effectively conveys the theme of illusion vs reality as Iago creates the â€Å"illusion† of being Othello’s honest friend.In comparison to â€Å"O†, Nelson also used characterisation and dramatic irony in shaping the theme illusion verses reality. However techniques differs greatly between the two text in order to suit its audiences. In â€Å"O†, Iago was being further conveyed through the character Hugo, who is a teenager boy and craves for everyone’s attention. â€Å"All my life, I always wanted to fly† is a voice over at both opening and closing of the movie indicating Hugo wanting to success and will be able to receive attention from his father and friends. Dramatic irony has been effectively used to engage audiences to reflect upon the nature of illusion versus reality.The conversation between Michael and Hugo, when Michael asked Hugo fo r advices about reputation. Hugo replied quickly â€Å"Like who gives a fuck about reputation, the only person you have to answer is yourself†, this quote is not only an indication of Hugo being a true Machiavellian villain, but it also highlights the theme of reality vs illusion. Shakespeare focused on values in integrity and reputation however to suit the 21st audiences Tim Blake Nelson have focused more on popularity. Hugo appeared to be a trustworthy friend in the film to both Oden and Michael and this is a representation of illusion verses reality.Tim Blake Nelson effectively presents this thought through the use of dramatic irony and mis en scene throughout the movie similarly to Shakespeare’s Othello. Despite the time and space, both composer explored the basic humanity by using similar techniques however it differs greatly to suit its contemporary audiences. Shakespeare’s â€Å"Othello† used literacy devices such as dramatic irony and soliloquies instead Tim Blake Nelson structured his film â€Å"O† using popular medium of cinema along with pop and opera music to create his universal themes.

Manchester United Economic Impact

c Impact Manchester United’s Economic Impact By Dalton Truax For Mike Pearson Sports Marketing & Management 28 February 2013 Introduction Manchester United is the most valuable sports club to exist so surely the club must make the city a lot of money every year, right? Soccer is the most popular sport in the world, and the most popular soccer club in the world is Manchester United. There is no denial that Manchester United has had a huge cultural impact as well as made many social contributions to its locale, but what about the team’s economic impact?Manchester United has influenced the local and regional economy in many ways, with most of them being exclusively positive. Some of the ways the club has influenced the local and regional economy are through networking, the economic impact of the supporters, employment, real estate, multiplier effects, and marketing of the city. Networks, Suppliers, Supporters I will first focus on Manchester United’s economic impact through networking, suppliers, and attracted supporters. First and foremost, there is most definitely an economic impact as a result of networking and suppliers.Penn’s model illustrates how a soccer club can play a role in regional development, using three possible network types. [1] In the first model (Figure 1), the club operates as an isolated business. The development effect is limited to the income that is spent again in the local economy, the income multiplier. The second model (Figure 2) embodies the situation in which the club has relations with other local companies like suppliers, subcontractors, and others, where the club is the heart of the economical development. Read also Twitter Case StudyThe given companies depend very much on the club. The last model (Figure 3) describes a network that does not exclusively depend on the soccer club. This is the case with the sponsor and merchandising network of Manchester United. Although the sponsor and merchandising networks are mostly situated on a national level, some of these companies can have an impact on the local scale. [2] Figure 1 [pic] Figure 2 [pic] Figure 3[pic] Manchester United has a worldwide fan base. There are supporters clubs all over the world because of their popularity.Supporters have an economical impact on the regional and local economy because of what they spend on transport, drinks, food, etc. A study on Manchester United supporters shows us that a supporter spends an average of $25 on drinks, food, and other products per match. [3] If the supporters are from outside the region, they bring in money to the local economy. We have to take into account that the soccer related expens es by local supporters are not an extra input for the local economy. A very limited number of seats are reserved for the supporters of the opposing team. 0% of the supporters are local, 40% are from the northwest region outside of the local community, and from outside the regional community are another 30%. [4] This means that a total of 70% of the supporters can generate a positive economic impact for the local economy, and 30% can do this for the regional economy. There were 68,187 seats in Old Trafford in 2010 and around 40 matches a year. This results in a yearly net income of $4,221,560,700. On a regional scale, this results in $18,092,403,000 every year. 5] These supporters’ expenditures end up especially in the hands of the hotel and catering industry in the immediate area around the stadium. Hotels are almost always full on the day of games. The direct employment by the club also has an economical impact, which is magnified by the income multiplier. The players may al so influence the real estate market quite a bit; players often invest in residential developments and other real estate operations. It is assumed that the presence of soccer players in a neighborhood can add to the attraction of that neighborhood.Some real estate agencies even try to attract soccer players to luxurious neighborhoods just so that they can attract other wealthy people. [6] National Image Because of globalization, cities and regions worldwide have entered into a struggle for investment, tourism, and development. Marketing of the city is a very important tool when it comes to this. The question is which role sports, and more specifically soccer clubs like Manchester United, can play here. Entertainment is playing a bigger and bigger role in society as the years go by. So, how does the government view Manchester United in this matter?The government acknowledges the important role Manchester United plays as a catalyst for marketing of the city. They regard Manchester Unit ed mainly as a publicity channel for the region to attempt to attract tourists, as well as companies to the region. Trafford Metropolitan Borough Council also uses the presence of Manchester United to further develop the area around the club. They have a long-term vision for the development of Old Trafford, the team’s historic stadium. The role of Manchester United in attracting businesses is very significant.The club can attract businesses itself but can only do this for businesses that benefit from the actual matches taking place, such as pubs and hotels. On the other hand, the club functions as a publicity channel for the region. It draws attention, and is always a good way to start a conversation. In this way, the club has indirectly made a large contribution to bring businesses and investments to the region. The brand name is known all over the world and puts Manchester on the map. Therefore it is of extreme importance that the club keeps a positive image and identity fo r the city.Conclusion We can conclude that Manchester United does not only have a cultural importance, but that they can also make an economical contribution by networking, the impact of supporters on surrounding businesses, and the role they might play in marketing for the city and regional development. The economic impact of Manchester United is vast and in many ways cannot even be described in numbers. ———————– [1]https://aow. kuleuven. be/geografie/toekomstigestudenten/onderzoek/vandenbroucke/fig1b_penn_model. gif [2]http://aow. kuleuven. e/geografie/toekomstigestudenten/onderzoek/vandenbroucke/index. html [3]http://www. foxbusiness. com/personal-finance/2011/12/20/5-pricey-nfl-stadiums-that-will-sack-your-wallet/ [4]http://www. telegraph. co. uk/sport/football/competitions/premier-league/9795377/Twitter-map-finally-reveals-exactly-where-Manchester-United-fans-live. html [5]http://www. bloomberg. com/news/2012-02-21/manches ter-united-quarterly-profit-rises-31-on-finance-costs-sponsors. html [6]http://lifestyle. ezinemark. com/luxurious-houses-of-premier-league-players-773659074564. html

Correlation Between Network And Network - 1546 Words

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